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In Situ Detection of DNA Damage Methods and Protocols
In Situ Detection of DNA Damage Methods and Protocols
Didenko Vladimir V. (Didenko Vladimir V.)
SKU: VV1609002 ISBN: 9780896039520 Pages: 336 Hardback year: 2002 Publicher: Springer (Springer) Price: $324.78 ship 24-25
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| In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in si |